Post by gradenjurare on May 21, 2019 6:54:52 GMT 3
Main category \ Education
Sub category \ Science
Developer \ CompOmics
Filesize \ 113459
Title \ PeptideShaker
hideuri.com/xyNJ1Q
1.16.40 PeptideShaker
Data analysis and interpretation remain major logistical challenges when attempting to identify large numbers of protein phosphorylation sites by nanoscale reverse-phase liquid chromatography/tandem mass spectrometry (LC-MS/MS) (Supplementary Figure 1 online). In this report we address challenges that are often only addressable by laborious manual validation, including data set error, data set sensitivity and phosphorylation site localization. We provide a large-scale phosphorylation data set with a measured error rate as determined by the target-decoy approach, we demonstrate an approach to maximize data set sensitivity by efficiently distracting incorrect peptide spectral matches (PSMs), and we present a probability-based score, the Ascore, that measures the probability of correct phosphorylation site localization based on the presence and intensity of site-determining ions in MS/MS spectra. We applied our methods in a fully automated fashion to nocodazole-arrested HeLa cell lysate where we identified 1,761 nonredundant phosphorylation sites from 491 proteins with a peptide false-positive rate of 1.3%. FEATURE IMPROVEMENT: Replaced uses of "\n" with tProperty("parator"), making the export less system dependent. LIBRARY UPDATE: Updated utilities to version 3.3.42, fixing an issue with unknown protein expect values in X!Tandem files. Name PeptideShaker oxyHost= oxyPort= NEW FEATURE: Added simple data filters for the Plotting Dialog.
Official:
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Recomended on iMac Pro macpkg.icu/?id=61024&kw=RsrWG.PeptideShaker.v.1.16.44.tar.gz | 135016 kb |
Updated to 10.11.5 macpkg.icu/?id=61024&kw=vers.1.18.40-PeptideShaker-HN7Bk.app | 124804 kb |
Moreover, PeptideShaker is divided into eight tabs covering aspects of the analysis: FEATURE IMPROVEMENT: Improved automatic spectrum annotation. cdfread Changes in PeptideShaker 1.7.6 (February 12. 2016): LIBRARY UPDATE: Updated utilities to version 3.43.10. Memory Issues II - Using more than 2GB of memory requires the installation of 64 bit Java. 64 bit Java is downloaded from the same place as the 32 bit version: Java. Note that 64 bit Java can only be used on 64 bit operating systems! It’s a fast and powerful app that can pull in video from a local file or a YouTube link, but you can also use it to stitch together images or record something on your Mac screen or with your smartphone camera. It has a feature-packed customization panel for extending or shortening length, cropping, changing file sizes, and tinkering with overlays and loops. There’s no better tool out there for what GIF Brewery 3 offers. platform for creating MS/MS data analysis workflows using scripts [13]
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